Human adipose-derived stem cells are the easiest adult stem cells to access, harvest, and isolate, providing the largest supply of autologous stem cells for clinical application1–4. As stem cells, hASCs have the ability to self-renew, differentiate, and suppress inflammation. These cells are being used along with other components of the stromal vascular fraction (SVF) to treat autoimmune conditions, inflammatory diseases, and specific injuries. In clinical trials, these cells are being isolated and expanded to treat a similar set of health conditions with a more targeted interest in regeneration. In addition to these on-going clinical studies there is also research being performed to generate tissue patches that can aid in the repair of significant injury to tissue including heart and skeletal muscle. With the interest in using isolated stem cells in cell-based treatments and in areas of tissue engineering and regenerative medicine it is critical that we find efficient and clinically relevant methods for maintaining and differentiating stem cells. To accomplish this, the proposed project brings together an interdisciplinary, university-industry collaborative team consisting of investigators from Biological Sciences and Biomedical Engineering at Louisiana Tech University, Medicine at Tulane University, and LaCell LLC, a Louisiana based biotechnology company that focuses on promoting a wider usage of primary human stromal/stem cell cultures as a more reliable, clinically relevant stem cell source.